Scindeks Assistant SCIndeks Assistant
Vol. 80 No. 11 (2023)

Cellular cryobiology – a review of basic concepts and “operating design” of cryopreserved cells

DOI: 10.2298/VSP230711044B
Bela Balint
Serbian Academy of Sciences and Arts, Department of Medical Sciences, Belgrade, Serbia; University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia
Mirjana Pavlović
Department of Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, USA
Džihan Abazović
Biocell Hospital, Department for Regenerative Medicine, Belgrade, Serbia
Sanja Toroman
SJOG Midland Hospital, Midland, Perth, Western Australia
Radica M. Grubović Rastvorčeva
Institute for Transfusion Medicine of RNM, Skopje, North Macedonia; Goce Delčev University, Faculty of Medical Sciences, Štip, North Macedonia
Marija Dinić
University Clinical Center of Serbia, ††Department of Apheresis Belgrade, Serbia
Milena Todorović Balint
University Clinical Center of Serbia, Clinic for Hematology, Belgrade, Serbia; University of Belgrade, Faculty of Medicine, Belgrade, Serbia
PDF

Published

2023-11-30

Section

Review Paper

How to Cite

1.
Balint B, Pavlović M, Abazović D, Toroman S, Grubović Rastvorčeva RM, Dinić M, et al. Cellular cryobiology – a review of basic concepts and “operating design” of cryopreserved cells. Vojnosanit Pregl [Internet]. 2023 Nov. 30 [cited 2026 Sep. 20];80(11). Available from: https://test.aseestant.ceon.rs/index.php/vsp/article/view/45442
ACS APA ABNT Chicago Harvard IEEE Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX

Keywords

cryopreservation
cryoprotective agents
cryoinjury
stem cells
platelets
DOI: 10.2298/VSP230711044B

References

Armitage WJ. Metabolism and physiology of cells at low tem-peratures. In: Smit Sibinga CT, Das PC, Meryman HT, editors. Cryopreservation and low temperature biology in blood trans-fusion. Developments in Hematology and Immunology, vol 24. Boston, MA: Springer; 1990. p. 1–10.

Pegg DE. Principles of cryopreservation. Methods Mol Biol 2007; 368: 39–57.

Jang TH, Park SC, Yang JH, Kim JY, Seok JH, Park US, et al. Cryopreservation and its clinical applications. Integr Med Res 2017; 6(1): 12–8.

Meryman HT. Cryopreservation of living cells: principles and practice. Transfusion 2007; 47(5): 935–45.

Pavlovic M, Balint B. Stem Cells and Tissue Engineering. New York: Springer Science & Business Media; 2012. p. 154.

Polge C, Smith AU, Parkes AS. Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature 1949; 164(4172): 666.

Lovelock JE, Bishop MW. Prevention of freezing damage to liv-ing cells by dimethyl sulfoxide. Nature 1959; 183(4672): 1394–5.

Meryman HT. Mechanics of freezing in living cells and tissues. Science 1956; 124(3221): 515–21.

Rowe AW, Rinfret AP. Controlled rate freezing of bone mar-row. Blood 1962; 20(5): 636.

Mazur P. Theoretical and experimental effects of cooling and warming velocity on the survival of frozen and thawed cells. Cryobiology 1966; 2(4): 181–92.

Whaley D, Damyar K, Witek RP, Mendoza A, Alexander M, Lakey JR. Cryopreservation: An overview of principles and cell-specific considerations. Cell Transplant 2021; 30: 963689721999617.

Uhrig M, Ezquer F, Ezquer M. Improving cell recovery: freezing and thawing optimization of induced pluripotent stem cells. Cells 2022; 11(5): 799.

Elliott GD, Wang S, Fuller BJ. Cryoprotectants: A review of the actions and applications of cryoprotective solutes that modulate cell recovery from ultra-low temperatures. Cryobiology 2017; 76: 74–91.

Balint B. Stem and progenitor cell harvesting, extracorporeal “graft engineering” and clinical use – initial expansion vs. cur-rent dilemmas. Clin Appl Immunol 2006; 5(1): 518–27.

Balint B, Ljubenov M, Stamatović D, Todorović M, Pavlović M, Ostojić G, et al. Stem cell harvesting protocol research in autologous transplantation setting: large volume vs. conventional cytapheresis. Vojnosanit Pregl 2008; 65(7): 545–51.

Balint B, Todorovic Balint M, Urosevic I, Pavlovic M. Stem cell transplant: from cell harvesting to cryopreservation. Med Pregl 2017; 70(Suppl 1): 41–5.

Balint B, Ivanovic Z, Petakov M, Taseski J, Jovicić G, Stojanović N, et al. The cryopreservation protocol optimal for progenitor re-covery is not optimal for preservation of marrow repopulating ability. Bone Marrow Transplant 1999; 23(6): 613–9.

Skoric D, Balint B, Petakov M, Sindjic M, Rodic P. Collection strategies and cryopreservation of umbilical cord blood. Trans-fus Med 2007; 17(2): 107–13.

Balint B, Vucetić D, Trajković-Lakić Z, Petakov M, Bugarski D, Brajusković G, et al. Quantitative, functional, morphological and ultrastructural recovery of platelets as predictor for cryopreservation. Haematologia (Budap) 2002; 32(4): 363–75.

Balint B, Paunovic D, Vucetic D, Vojvodic D, Petakov M, Trkuljic M, et al. Controlled-rate versus uncontrolled-rate freezing as predictors for platelet cryopreservation efficacy. Transfusion 2006; 46(2): 230–5.

Stolzing A, Naaldijk Y, Fedorova V, Sethe S. Hydroxyethylstarch in cryopreservation - mechanisms, benefits and problems. Transfus Apher Sci 2012; 46(2): 137–47.

Balint B, Stamatović D, Todorović M, Jevtić M, Ostojić G, Pavlović M, et al. Stem cells in the arrangement of bone marrow repopulation and regenerative medicine. Vojnosanit Pregl 2007; 64(7): 481–4.

Balint B, Pavlovic M, Todorovic M. Stem cells: Haemobiology and clinical data summarising: a critical review. Scr Med 2020; 51(4): 261–71.

Balint B, Pavlovic M, Todorovic M. From nucleated to ex vi-vo manipulated stem cells – an updated biological and clinical synopsis. Med Word 2020; 1(1): 1–8.

Balint B, Pavlovic M, Marković O, Borovic S, Todorovic M. A stem cell overview – from evolving hemobiological concepts to (au-to)grafting in clinical practice. Serb J Med Chamber 2022; 3(2): 135–48.

Balint B, Stanojevic I, Todorovic M, Stamatovic D, Pavlovic M, Voj-vodic D. Relative frequency of immature CD34+/CD90+ sub-set in peripheral blood following mobilization correlates close-ly and inversely with the absolute count of harvested stem cells in multiple myeloma patients. Vojnosanit Pregl 2017; 74(11): 1071–7.

Zeng G, Hu Y, Hu X, Zeng W, Liang X, Liu Y, et al. Cryopreservation of peripheral blood mononuclear cells us-ing uncontrolled rate freezing. Cell Tissue Bank 2020; 21(4): 631–41.

Setia RD, Arora S, Handoo A, Choudhary D, Sharma SK, Khandel-wal V, et al. Outcome of 51 autologous peripheral blood stem cell transplants after uncontrolled-rate freezing ("dump freez-ing") using -80°C mechanical freezer. Asian J Transfus Sci 2018; 12(2): 117–22. doi: 10.4103/ajts.AJTS_42_17

Halle P, Tournilhac O, Knopinska-Posluszny W, Kanold J, Gembara P, Boiret N, et al. Uncontrolled-rate freezing and storage at -80 degrees C, with only 3.5-percent DMSO in cryoprotective so-lution for 109 autologous peripheral blood progenitor cell transplantations. Transfusion 2001; 41(5): 667–73. doi: 10.1046/j.1537-2995.2001.41050667.x

Todorović-Balint M, Bila J, Balint B, Jeličić J, Djunić I, Antić D, et al. Influence of applied CD34+ cell dose on the survival of Hodgkin's lymphoma and multiple myeloma patients following autologous stem cell transplants. Vojnosanit Pregl 2020; 77(8): 844–51.

Abrahamsen JF, Bakken AM, Bruserud Ø. Cryopreserving human peripheral blood progenitor cells with 5-percent rather than 10-percent DMSO results in less apoptosis and necrosis in CD34+ cells. Transfusion 2002; 42(12): 1573–80.

Cai J, Weiss ML, Rao MS. In search of "stemness". Exp Hematol 2004; 32(7): 585–98.

Dianat-Moghadam H, Sharifi M, Salehi R, Keshavarz M, Shahgolzari M, Amoozgar Z. Engaging stemness improves cancer immunotherapy. Cancer Lett 2023; 554: 216007.

Al-Azab M, Idiiatullina E, Safi M, Hezam K. Enhancers of mes-enchymal stem cell stemness and therapeutic potency. Biomed Pharmacother 2023; 162: 114356.

Cai J, Chen H, Xie S, Hu Z, Bai Y. Research progress of totipotent stem cells. Stem Cells Dev 2022; 31(13–14): 335–45.

Liesveld JL, Sharma N, Aljitawi OS. Stem cell homing: From physiology to therapeutics. Stem Cells 2020; 38(10): 1241–53.

Quesenberry PJ, Wen S, Goldberg LR, Dooner MS. The universal stem cell. Leukemia 2022; 36(12): 2784–92.

Slack JMW. What is a stem cell? Wiley Interdiscip Rev Dev Biol 2018; 7(5): e323.

Samperio Ventayol P, Bartfeld S. Immune cell-stem cell interac-tions in regeneration and repair: who's calling the shots? De-velopment 2022; 149(8): dev200228.

Rudolph KL. Stem cell aging. Mech Ageing Dev 2021; 193: 111394.

Lee DA, Knight MM, Campbell JJ, Bader DL. Stem cell mecha-nobiology. J Cell Biochem 2011; 112(1): 1–9.

Chen X, Tang K, Li X, Zhang C, Xin Y, Li K, et al. Biomechan-ics of cancer stem cells. Essays Biochem 2022; 66(4): 359–69.

Hoang DM, Pham PT, Bach TQ, Ngo ATL, Nguyen QT, Phan TTK, et al. Stem cell-based therapy for human diseases. Signal Transduct Target Ther 2022; 7(1): 272.